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Collin County Community College Acids Bases and PH Report Sheet Biology Lab

Question Description

Download the report sheet, complete it, and then submit it via Blackboard email as an attachment.Do not change the format.

All blue shaded areas require answers.


I.Using indicators to measure pH

Experiment (at home lab)

Making a set of standards (5 ml solution + 5 ml cabbage extract)

pH

substance

color

2

lemon juice

3

vinegar

5(optional)

rainwater

7

tap water

11.5

ammonia or soapy water

Determining the pH of some solutions (5 ml unknown + 3 ml cabbage extract)

Unknowns

estimate pH value (not color)

7up®

grapefruit juice

bleach

II.The pH Meter and pH paper

Experiment (dry lab)

Measuring pH using a pH meter and pH paper.

Solution

pH meter

pH paper (estimate pH value not color)

distilled water

apple juice

vinegar

carbonated soft drink

coffee

detergent water

milk

Which solution is most acidic?

Which solution is most basic?

Which measurement is more accurate, pH meter or pH paper?

III.Buffers

Experiment (dry lab)

Determine the buffering capacity of a solution.

pH of Solution A

0 ml

1ml

2ml

3ml

4ml

5ml

6ml

7ml

8ml

9ml

10ml

HCl

NaOH

pH of Solution B

0 ml

1ml

2ml

3ml

4ml

5ml

6ml

7ml

8ml

9ml

10ml

HCl

NaOH

Plot both A and B solutions, A in red and B in blue. (This can be done using Microsoft insert shapes or manually graph, scan and embed image into your report).

Which is the better-buffered solution, A or B?

The better-buffed solution will resist a change in pH.

IV.How Effective are Stomach Antacids?

Experiment (dry lab)

Neutralization capacity of antacids

Flasks

Initial pH

Final pH

control

Rolaids

Alka-Seltzer

Tums

Which antacid neutralized the most acid?

Questions

1.

Would you have been able to determine the pH of the three unknowns using the cabbage juice if you had not made your standards (the test tubes containing each of the buffers and the cabbage juice)?

Yes or no.

2.

Suppose that you were given a sample that had a pH of 6.5 but you were unaware of the pH.

a. Can you perceive a problem that would arise if you attempted to determine the pH of this sample using either anthocyanins or pH paper?

b. What about using a pH meter?

3.

a. Is the use of anthocyanins qualitative or quantitative?

b. is the use of a pH meter qualitative or quantitative?

4.

Which measurement would you consider to be a most reliable measurement; anthocyanins, pH paper or pH meter?

End of Lab J

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