California National University Patient Symptoms and Signs and Test Results Questions

Question Description

A patient presented to the emergency room with a dog bite on the side of her face. The dog was up to date with all its shots.

No fever, normal blood pressure, and cardiovascular, respiratory, central nervous, and gastrointestinal systems were observed to be normal.

A culture was performed on the laceration on the left cheek.

Results:

Aerobic culture on blood agar medium of the dog bite wound on the face showed two types of colonies of organisms. A second culture of both the colonies was performed

Culture from the smaller colony done on blood agar showed 1-2 mm smooth gray non-hemolytic mucoid colonies. The organism did not grow on Mac-Conkey agar or EMB agar

Subculture from the larger colony done on EMB showed large pale cream mucoid colonies. The organism grew well on MacConkey agar with no color change..

Biochemical tests:

TESTS PERFORMED	REACTION TESTED	RESULTS
NO₃	Reduction of nitrates to nitrites	POSITIVE
TRP	Indole production	POSITIVE
GLUCOSE	Acid production from Glucose	POSITIVE
ADH	Arginine hydrolysis	NEGATIVE
UREA	Urease	NEGATIVE
ESCULIN	Hydrolysis (b-glucosidase)	NEGATIVE
GELATIN	Hydrolysis (protease)	NEGATIVE
PNPG	b-galactosidase	NEGATIVE

the oxidase test, catalase test, ornithine decarboxylase test and the indole test were all positive.

Smaller colony:

Based on the results on blood agar and MacConkey and EMB agar, describe the organism. (There are only two characteristics you can describe, you do not have to name an organism.)

Larger colony:

Based on the results on MacConkey and EMB, what do you expect to see when you Gram stain the organism.
What other biochemical characteristic is demonstrated by the results on MacConkey and EMB

Describe what each of the positive biochemical tests

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