Activity 1: Determining the Reaction Rate in the Presence or Absence of Cellobiase

Data Table 1: Comparison of Reaction Cuvettes to Standard Cuvettes.

Time (minutes)	Cuvette	Standard that is Most Similar	Amount of p-nitrophenol (nmol)
0	START	S1
8	END	S1
1	E1	S2
2	E2	S3
4	E3	S4
6	E4	S5
8	E5	S5

Activity 1 Questions

Describe any changes you observed in the enzyme reaction and control reaction conical tubes during the time that the reaction was occurring?
What happened to the solution in each cuvette after you added the enzyme/substrate mixture to the stop solution that was in the cuvette?
Describe the amount of product produced in the enzyme-catalyzed reaction compared to the control where no enzyme was added.
If you took another time point at 15 minutes, do you think more product would be produced than at 8 minutes? Explain your answer.
Is the rate of product formation constant over time? (Hint: Is the slope of the line constant or does it change over time?)

Activity 2: Determining Effects of Temperature on Cellobiase Activity

Data Table 2: Determination of ?-nitrophenol produced at three different temperatures based on ?-nitrophenol standards

Temperature	Standard that is most similar	Amount of ?-Nitrophenol Produced (nmol)
0C	S2
Room temp	S3
37C	S5

At what temperature does this enzyme work best? Explain how you know this.

Why do chemical reactions occur faster at higher temperatures? Is that true for all enzymes, and unlimited temperature values?

Why do most enzymatic reactions slow down at extremely high temperatures?

Activity 3: Determining Effects of pH on Cellobiase Activity

Data Table 3: Determination of ?-nitrophenol produced at three different pHs based on p-nitrophenol standards

pH	Standard that is most similar	Amount of p-Ntrophenol Produced (nmol)
5.0	S3
6.3	S2
8.6	S1

Activity 3 Questions

A. At what pH does this enzyme work best? Explain how you know.

B. Why do most enzymatic reactions slow down at extremely high or low pH values?

C. In what type of environment might an organism that produces this enzyme live? Explain your reasoning

Activity 4: Summary

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