Collin County Community College Osmosis and Diffusion Bio Lab

Question Description

OSMOSIS AND DIFFUSION REPORT SHEET

Download the report sheet, complete it, and then submit it via Blackboard email as an attachment.Do not change the format.

All blue shaded areas require answers.

Osmosis

Experiment (dry lab)

There are 3 diagrams of animal cells (a, b, c) and 3 diagrams of plant cells (a, b, c) in solutions of varying tonicity. Label the diagrams as isotonic solution, hypertonic solution, or hypotonic solution.

Animal cells:
(a)
(b)
(c)
Plant cells:
(a)
(b)
( c)

Effect of temperature and size
Effect of solute concentration
Differential permeability of membranes
Diffusion and osmosis in living cells

Observe the agar plate at 25°C, 30 min.

Experiment (dry lab)

Potassium permanganate at 25°C		Janus Green at 25°C
Time	Distance in mm	Time	Distance in mm
30 min		30 min

Potassium permanganate (294 Daltons) and Janus Green (511 Daltons) dyes

Which dye has greater diffusion, potassium permanganate or Janus Green?
Why?

Observe the agar plate at 5°C, 30 min.

Experiment (dry lab)

Potassium permanganate at 5°C		Janus Green at 5°C
Time	Distance in mm	Time	Distance in mm
30 min		30 min

Potassium permanganate (294 Daltons) and Janus Green (511 Daltons) dyes

Which dye has greater diffusion potassium permanganate or Janus Green?
Why?

Compare the agar plates at 5°C and 25°C.

Experiment (dry lab)

Which agar plate shows greater diffusion, 5°C or 25°C?
Why?

Effect of temperature on diffusion of iodine

Experiment (at home lab)

QUESTIONS

Temperature of your cups:
Is the food coloring evenly distributed in each?
Explain.

Experiment (dry lab)

In the weight change column, record if the bag should increase in weight, decrease in weight, or stay about the same.

#	Bag contents	Beaker contents	Weight change
1	10 ml Distilled water	200 ml Distilled water
2	10 ml 10% sucrose	200 ml Distilled water
3	10 ml 20% sucrose	200 ml Distilled water
4	10 ml 40% sucrose	200 ml Distilled water
5	10 ml Distilled water	200 ml 40% sucrose
Which bag should gain the most weight?

Experiment (dry lab)

Test for	Is test positive or negative?
Starch
Protein
Chloride ion
Sulfate ion

Which ones (starch, protein, chloride ions, sulfate ions) moved through the dialysis tubing and into the beaker?
Why did some not move from the bag into the beaker?

Experiment (dry lab)

A.Osmosis with red blood cells

	Describe the blood cells.
0.9% NaCl , Normal blood cells
10% NaCl, Crenated cells

1. What solution causes cells to appear this way? Isotonic, hypertonic or hypotonic solution.	2. What solution causes cells to appear this way? Isotonic, hypertonic or hypotonic solution.

Osmosis with plant cells

	Describe the plant cells.
0% NaCl, Turgid cells
20% NaCl, Plasmolyzed cells

1. What solution causes cells to appear this way? Hypertonic or hypotonic solution.	2. What solution causes cells to appear this way? Hypertonic or hypotonic solution.

Diffusion in cucumber cells

Experiment (at home lab)

QUESTIONS

1. When comparing the firmness of the cucumber slices, how would you describe each, firm (turgid cells) or soft (plasmolyzed cells)?
Cup 1
Cup 2
Cup 3

2. What caused one to be firm and the other to be soft?

Explain.

End of Lab J

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