Central Florida Parallel and Serial Dilution Calculations Chemistry Lab Experiment

Question Description

Here are some specific information you must include in this pre-lab:

1. You will need to show how to preform parallel and serial dilution calculations. Assume the stock concentration of red dye #40 is 0.1 M. Use any concentration within the range of 0.01 M to 0.1 M.

Prepare five different concentrations (total volume can range from 10-50 mL) using parallel and serial dilutions.

2. Describe how to use the UV-Vis spectrometer. What will you use as a blank?

NOTE!! To help you understand serial dilutions, I have added the link to aYouTube video

Prelab Rubric


Criteria	Ratings	Pts
This criterion is linked to a Learning OutcomeTitle, your name, date of the experiment, course and section number, and experiment number.		2.0 pts
This criterion is linked to a Learning OutcomeAim: State the aim of the experiment in your own words, usually around 2-4 sentences. A clear statement of the overall purpose for performing the experiment, i.e. what is / are the goal(s) to be achieved and what idea(s) are you to learn during the duration of the project?		3.0 pts
This criterion is linked to a Learning OutcomeMaterials: Write all equipment or glassware with specific sizes needed. (For example: 100 mL volumetric flask)		2.0 pts
This criterion is linked to a Learning OutcomeChemicals/Hazards : In a table, list all chemicals needed along with specific hazard information and other information necessary for the experiment (e.g., chemical name, chemical formula, molecular formula weight, physical appearance).For example: Chemical Name, Chemical Formula, Chemical Weight, Physical Appearance, Specific Hazard and Additional Notes. Include important safety information regarding all chemicals that may be used during the experiment. MSDS information can be found online.		3.0 pts
This criterion is linked to a Learning OutcomeMethods and Procedures: Based on the descriptions of the experiment, in your own words, design and describe the steps for the experiment. Make sure not to copy of the exact procedures from the lab manual.It should be written in your own words in a step-wise manner (diagrams or flowchart are encouraged).		5.0 pts
This criterion is linked to a Learning OutcomeCalculations : Provide any calculations necessary for the lab ahead of time.For example: what is the mass in grams required to prepare 250 mL of a 0.5 M NaCl solution?		5.0 pts
Total Points: 20.0

In this section, you will need to submit a typed Word document answering the questions below. Make sure to include a title, your name, section number, the date, and ALL the calculations and data tables involved with the assignment.

1. Answer all the post lab summary questions provided on page 21 in the lab manual.There are five questions and each is worth 6 points, to a total of 30 points.

2. Use the absorbance and concentration data provided below to create two calibration plots, one for serial dilutions and the other for parallel dilutions (10 points). The calibration plots will need to have: correct title of the graph, x and y-axis labeling, linear equation, R-squared value, and the slope should be displayed on the graph. Plot the two graphs using Excel. Use the x-y scatter plot without a line and then add a trendline. If you need additional help with this, please research online or attend my office hours.

Absorbance Values from Serial Dilution

Sample	Concentration (in M)	Absorbance @ 465 nm Trial 1	Absorbance @ 465 nm Trial 2	Absorbance @ 465 nm Trial 3	Average Absorbance
A	1.25×10-4	1.213	1.096	1.243
B	6.25×10-5	0.837	0.836	0.865
C	3.125×10-5	0.492	0.494	0.492
D	1.5625×10-5	0.265	0.27	0.263
E	7.8125×10-6	0.146	0.153	0.148

* Scroll to the right to see all the data in the table

Absorbance Values from Parallel Dilution

Sample	Concentration (M)	Absorbance @ 465 nm Trial 1	Absorbance @ 465 nm Trial 2	Absorbance @ 465 nm Trial 3	Average Absorbance
A	2.5×10-5	0.387	0.394	0.397
B	5×10-5	0.725	0.733	0.728
C	7.5×10-5	0.912	0.924	0.918
D	1×10-4	1.113	1.152	1.127
E	1.25×10-4	1.192	1.207	1.195

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